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| Registros recuperados: 32 | |
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| Cheret, Romuald; Delbarre Ladrat, Christine; De Lamballerie Anton, Marie; Verrez-bagnis, Veronique. |
| Post mortem tenderization is one of the most unfavourable quality changes in fish muscle and this contrasts with muscle of mammalian meats. The tenderization can be partly attributed to the acid lysosomal cathepsins and cytosolic neutral calcium-activated calpains. In this study, these proteases from fish and bovine muscles were quantified and compared. The cathepsin B and L activities were in more important amounts in sea bass white muscle than in bovine muscle. On the other hand, cathepsin D activity was 1.4 times higher in meat that in fish muscle, while cathepsin H was negligible in both muscles. Calpain activities were similar in both types of muscle. Moreover, calpastatin (calpain endogenous inhibitor) level is 3.9 times higher in sea bass white... |
| Tipo: Text |
Palavras-chave: Meat; Fish; Protease; Cathepsin; Calpain. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3646.pdf |
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| Passerini, Delphine; Fecamp, Florian; Marchand, Laetitia; Kolypczuk, Laetitia; Bonnetot, Sandrine; Sinquin, Corinne; Verrez-bagnis, Veronique; Hervio Heath, Dominique; Colliec Jouault, Sylvia; Delbarre Ladrat, Christine. |
| In the marine environment, biofilm formation is an important lifestyle for microorganisms. A biofilm is comprised of cells embedded in an extracellular matrix that holds them close together and keeps the biofilm attached to the colonized surface. This predominant lifestyle and its main regulation pathway, namely quorum-sensing (QS), have been shown to induce specific bioactive metabolites. In this study, we investigated the biofilm formation by two marine bacteria belonging to the Vibrio species to discover potentially innovative bioactive compounds. We proposed a protocol to isolate biofilm extracts, to analyze their biochemical composition, and to compare them to planktonic cell extracts. Cells were grown attached to a plastic surface; extracts were... |
| Tipo: Text |
Palavras-chave: Vibrio; Biofilm; Polysaccharide; Quorum-sensing; Activity. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00592/70381/68461.pdf |
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| Griffiths, Andrew M.; Sotelo, Carmen G.; Mendes, Rogerio; Perez-martin, Ricardo I.; Schroeder, Ute; Shorten, Marc; Silva, Helena A.; Verrez-bagnis, Veronique; Mariani, Stefano. |
| Mislabelling of food products has recently received a great deal of public scrutiny, but it remains unclear exactly what methods are being utilised in laboratories testing the authenticity of foods. In order to gain insight into the specific area of the analysis of seafood, a questionnaire focusing on the taxonomic groups typically analysed and the techniques utilised was sent to over one hundred accredited laboratories across the UK, Ireland, Spain, Portugal, France and Germany. Forty-five responded positively, demonstrating significant differences in both the species analysed and methods utilised among the countries included in the survey. Indeed, a diversity of methods was employed across laboratories and efforts to harmonise and/or standardise testing... |
| Tipo: Text |
Palavras-chave: Food testing; Forensically informative nucleotide sequencing; Species identification; DNA barcoding; Fisheries. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00188/29918/28383.pdf |
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| Bojolly, Daline; Doyen, Perine; Le Fur, Bruno; Christaki, Urania; Verrez-bagnis, Veronique; Grard, Thierry. |
| Bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares) are among the most widely used tuna species for canning purposes. Not only substitution but also mixing of tuna species is prohibited by the European regulation for canned tuna products. However, as juveniles of bigeye and yellowfin tunas are very difficult to distinguish, unintentional substitutions may occur during the canning process. In this study, two mitochondrial markers from NADH dehydrogenase subunit 2 and cytochrome c oxidase subunit II genes were used to identify bigeye tuna and yellowfin tuna, respectively, utilizing TaqMan qPCR methodology. Two different qPCR-based methods were developed to quantify the percentage of flesh of each species used for can processing. The first one... |
| Tipo: Text |
Palavras-chave: Tuna; Authentication; Quantification; Canned products; TaqMan; QPCR; Species identification; Bigeye tuna (Thunnus obesus); Yellowfin tuna (Thunnus albacares). |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00371/48217/48403.pdf |
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| Cheret, Romuald; Delbarre Ladrat, Christine; Verrez-bagnis, Veronique; De Lamballerie, Marie. |
| Calpains (calcium-activated neutral proteases) of sea bass (Dicentrarchus labrax L.) muscle may participate in the degradation of muscle tissue during postmortem storage. These enzymes are regulated by calpastatin, their endogenous specific inhibitor. The objective of this study was to evaluate the changes encountered by the calpain system during the postmortem storage of fish muscle after high-pressure treatment. From 100 MPa, high-pressure treatment of purified calpains results in a loss of their activity as well as in the dissociation of the heterodimeric form. In muscle, the high-pressure processing decreases the initial activity of calpain. This loss in activity may be due to an inactivation by a change of structure. Initial calpastatin activity is... |
| Tipo: Text |
Palavras-chave: Postmortem denaturation; High pressure; Fish; Calpain system. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2786.pdf |
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| Cheret, Romuald; Chapleau, Nicolas; Delbarre Ladrat, Christine; Verrez-bagnis, Veronique; De Lamballerie, Marie. |
| High pressure is an innovative non-thermal food-preservation technology. We studied the effect of high-pressure treatment up-to 500 MPa for 5 min on physical characteristics of sea bass fillets after 0, 7, and 14 d of refrigerated storage.. Color results exhibited an increase of lightness and a slight change of hue, which might be imperceptible in cooked fish. High-pressure treatment induced a decrease of exudation and water-holding capacity Pressure treatment above 300 MPa provoked higher fish hardness after storage than in untreated sample, proving the ability of high pressure to improve textural, quality of chilled, stored fish fillet. These assessments were corroborated with microstructure observations. We showed that high-pressure treatment at 500 MPa... |
| Tipo: Text |
Palavras-chave: Preservation; Microstructure; Texture profile analysis; High pressure; Fish. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-734.pdf |
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| Verrez-bagnis, Veronique. |
| La récupération mécanique de pulpe de poisson à partir des résidus de filetage permet d’augmenter de 6 à 10 % le rendement en chair utilisable du cabillaud Cadus morhua. Mais pendant l'entreposage congelé, les proteines de la pulpe se dénaturent très rapidement, aussi l'emploi de substances (qu'on appelle cryoprotecteurs) qui ralentiraient, retarderaient ou inhiberaient partiellement la dénaturation des protéines a-t-il été envisagé. Afin de profiter des divers mécanismes d'action de chaque agent, il a paru intéressant de tester des mélanges de substances cryoprotectrices. |
| Tipo: Text |
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| Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/00137/24868/22956.pdf |
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| Calvo Dopico, D.; Mendes, R.; Silva, H.a.; Verrez-bagnis, Veronique; Pérez-martín, R.; Sotelo, C.g.. |
| This study aims to investigate the benefits associated with traceability and its relationship to quality. Market research has been conducted in Spain, Portugal, France, UK and Germany. The results show that the expected benefits for consumers related to traceability focus on food safety and quality. Relevant information about traceability should focus on the intrinsic quality attributes highly valued by consumers and food safety. Among them, it highlights the origin, the value of which has been highly appreciated by most consumers in different countries. As regards signalling, consumers rate labelling very favourably, which would have to offer all relevant information on the intrinsic quality attributes. The implementation of technology (e.g. QR) could... |
| Tipo: Text |
Palavras-chave: Labelling; Signal; Consumer behaviour; Traceability; Quality. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00349/46017/46901.pdf |
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| Nielsen, Einar E.; Cariani, Alessia; Mac Aoidh, Eoin; Maes, Gregory E.; Milano, Ilaria; Ogden, Rob; Taylor, Martin; Hemmer-hansen, Jakob; Babbucci, Massimiliano; Bargelloni, Luca; Bekkevold, Dorte; Diopere, Eveline; Grenfell, Leonie; Helyar, Sarah; Limborg, Morten T.; Martinsohn, Jann T.; Mcewing, Ross; Panitz, Frank; Patarnello, Tomaso; Tinti, Fausto; Van Houdt, Jeroen K. J.; Volckaert, Filip A. M.; Waples, Robin S.; Carvalho, Gary; Albin, Jan Ej; Vieites Baptista, Juan M; Barmintsev, Vladimir; Bautista, Jose M.; Bendixen, Christian; Berge, Jean-pascal; Blohm, Dietmar; Cardazzo, Barbara; Diez, Amalia; Espineira, Montserrat; Geffen, Audrey J.; Gonzalez, Elena; Gonzalez-lavin, Nerea; Guaniero, Ilaria; Jerome, Marc; Kochzius, Marc; Krey, Grigorius; Mouchel, Olivier; Negrisolo, Enrico; Piccinetti, Corrado; Puyet, Antonio; Rastorguev, Sergey; Smith, Jane P; Trentini, Massimo; Verrez-bagnis, Veronique; Volkov, Alexander; Zanzi, Antonella. |
| Illegal, Unreported and Unregulated fishing has had a major role in the overexploitation of global fish populations. In response, international regulations have been imposed and many fisheries have been 'eco-certified' by consumer organizations, but methods for independent control of catch certificates and eco-labels are urgently needed. Here we show that, by using gene-associated single nucleotide polymorphisms, individual marine fish can be assigned back to population of origin with unprecedented high levels of precision. By applying high differentiation single nucleotide polymorphism assays, in four commercial marine fish, on a pan-European scale, we find 93-100% of individuals could be correctly assigned to origin in policy-driven case studies. We show... |
| Tipo: Text |
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| Ano: 2012 |
URL: https://archimer.ifremer.fr/doc/00090/20085/17722.pdf |
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| Bautista, José M.; Verrez-bagnis, Veronique; Kourti, Naouma. |
| Taxonomy and systematics of most animal species have been described in sufficient detail to permit the classification of practically any organism and, in particular, may be of use in the identification of the species and subspecies for the fish trade and ecological concerns regarding fisheries. However, before FishTrace started there was a lack of fast reference tools that hindered the efficient identification and differentiation of teleosts required in fisheries management, biological and ecological research, and human consumption. This problem could affect several of the socio-economic activities related to Fisheries Policies and therefore, the constitution of a database for identification of fish species of commercial, ecological and zoological interest... |
| Tipo: Text |
Palavras-chave: Database; Fish; Genetics. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/rapport-6595.pdf |
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| Mariani, Stefano; Griffiths, Andrew M.; Velasco, Amaya; Kappel, Kristina; Jerome, Marc; Perez-martin, Ricardo I.; Schroeder, Ute; Verrez-bagnis, Veronique; Silva, Helena; Vandamme, Sara G.; Boufana, Belgees; Mendes, Rogerio; Shorten, Marc; Smith, Cat; Hankard, Elizabeth; Hook, Samantha A.; Weymer, Alice S.; Gunning, Daryl; Sotelo, Carmen G.. |
| Over the span of a decade, genetic identification methods have progressively exposed the inadequacies of the seafood supply chain, revealing previously unrecognized levels of seafood fraud, raising awareness among the public, and serving as a warning to industry that malpractice will be detected. Here we present the outcome of the latest and largest multi-species, transnational survey of fish labeling accuracy to date, which demonstrates an apparent sudden reduction of seafood mislabeling in Europe. We argue that recent efforts in legislation, governance, and outreach have had a positive impact on industry regulation. Coordinated, technology-based, policy-oriented actions can play a pivotal role in shaping a transparent, sustainable global seafood market... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00302/41323/40517.pdf |
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| Verrez-bagnis, Veronique; Soree, Marion; Passerini, Delphine; Kolypczuk, Laetitia; Marchand, Laetitia; Bonnetot, Sandrine; Fecamp, Florian; Lozach, Solen; Hervio Heath, Dominique; Delbarre Ladrat, Christine. |
| Vibrio spp. are ubiquitous marine bacteria that are ecologically and metabolically diverse members of planktonic and animal associated microbial communities. They encompass the ancient and well-studied human pathogen, Vibrio cholerae, and two other human pathogens, V. vulnificus and V. parahaemolyticus, as well as some less thoroughly characterized animal pathogens. Virulence is based on a wide diversity of mechanisms involved in motility and host colonization, in ability to persist and develop, and in damage generation. Polysaccharides may play major roles in virulence and are major components of extracellular polymeric matrix synthesized upon biofilm growth. They may also exhibit biological features, especially those similar to animal-derived... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00514/62605/66965.pdf |
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| Seesao, Y.; Audebert, C.; Verrez-bagnis, Veronique; Merlin, S.; Jerome, Marc; Viscogliosi, E.; Dei-cas, E.; Aliouat-denis, C. M.; Gay, Melanie. |
| Different methods were evaluated to extract DNA from pooled nematodes belonging to Anisakis, Contracaecum, Pseudoterranova and Hysterothylacium genera isolated from edible fish. Pooled DNA extraction is the first and compulsory step to allow the identification of a large number of samples through high-throughput DNA sequencing with drastic time and cost reductions. |
| Tipo: Text |
Palavras-chave: Anisakidae; High-throughput sequencing; Nematode; Pooled DNA extraction; Marine fish; COX2. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00189/30020/28607.pdf |
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| Lelievre, Stephanie; Verrez-bagnis, Veronique; Jerome, Marc; Vaz, Sandrine. |
| A better knowledge on spawning grounds of principal commercial fishes from North Sea is necessary for their monitoring. Fish egg taxonomic identification based on morphological characters cannot always be carried out effectively. This is particularly the case for a number of Gadidae fish species eggs (i.e. cod, haddock and whiting, and three rockling species) which have the same range of size and for which molecular-based techniques can help improving the accuracy of egg taxonomic identification, and hence the reliability of studies on spawning areas. In this study, formalin fixation, egg conservation and DNA extraction were optimized in order to develop a specific PCR-RFLP method. Based on the sequence of the cytochrome b gene of reference specimens, a... |
| Tipo: Text |
Palavras-chave: Fish eggs; PCR-RFLP; Cytochrome b gene; Geostatistical analyses; Interpolated map. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00005/11613/8259.pdf |
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| Registros recuperados: 32 | |
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